Adhesion Properties of Locally-Isolated Probiotic and Bacteriocinogcnic Lactic Acid Bacteria on Pig Intestines

Abstract

This study examined the adhesion property of Lactobacillus pentosus 3G3 on pig intestine by comparing it to a known probiotic agent, Lactobacillus casei 1Y9. Pig duodenum and middle colon were obtained, cut and washed, and inoculated with L. pentosus 3G3 and Lactobacillus casei 1Y9. Swabs were obtained from these intestines and were plated. After two days of incubation, colonies were counted and cfu/mL was computed. The population density of L. pentosus 3G3 on the duodenum and middle colon were 1.295xl07 cfu/mL and 3.727xl07 cfu/mL, respectively. For Lactobacillus casei 1Y9, adhesion to pig duodenum resulted to a population count of 1.229xl07 cfu/mL, while the calculated density on the middle colon was 7.41xl07 cfu/mL. The adhesion properties of Lactobacillus pentosus 3G3 was similar (p<0.05) to that of L. casei 1Y9 both in the duodenum and the colon, suggesting effective adhesion by the test organism to the pig intestine. Adhesion property was confirmed by scanning electron microscopy which showed that L. pentosus 3G3 colonies were able to adhere in the pig duodenum and colon.