dc.description.abstract |
Random Amplified Polymorphic DNA (RAPD) analysis was applied to
three previously recognized species of Oliva from the Visayas. Total
genomic DNA was extracted from the ethanol-preserved samples
following the method of Chomczynski et al. (1997) with some
modifications. To assay genetic polymorphisms, the genomic DNAs were
subjected to Polymerase Chain Reaction using one random primer.
Different RAPD fragment patterns were observed for the different species.
Analysis of the data using Popgene ver. 1.31 showed separation of the
samples into four discrete groups - O. vidua, O. amythestina, O. reticulata
and O. tigridella. |
en_US |